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滴定CFSE可了解最佳的CFSE用量,使增殖分析的染色最优化,因为如果CFSE太少会限制细胞分裂代数的检测,如果CFSE太多则会影响细胞活性和分裂。 最佳的染色浓度是使父代细胞荧光强度比未染色细胞高3~3.5个数量级。 这个帖子发布于 9 年零 100 天前,其中的信息可能已发生改变或有所发展。 像上图这种图怎么看? 图中的数字代表的是CFSE荧光强度位于这个范围内的细胞的百分比吗?这种图是不是只是看这个数值就好了? 那不同的峰的高低代表什么呢? 细胞数量? CFSE (carboxyfluorescein diacetate, succinimidyl ester 羧基荧光素双乙酸盐,琥珀酰亚胺酯) 被动散进入细胞,其本身是无色无荧光的,被细胞内的酯酶分解后产生高强度的荧光,该荧光产物与胞内氨基结合长时间留存于细胞内并可被乙醛固定。未结合的试剂与副产物扩散至胞外基质中,被洗去。 传代或胚胎.
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The crystal field stabilisation energy (cfse) is the gain in the energy achieved by preferential filling up of orbitals by electrons Click here👆to get an answer to your question ️ the crystal field splitting energy for octahedral deltacirc and It is usually less than or equal to 0
When it is equal to 0, the complex is unstable
The magnitude of cfse depends on the number and nature of ligands and the geometry of the complex Three electrons are in lower t2g level. 求助:OT-1分选出的naive CD8 T跟DC细胞和处死的肿瘤细胞共孵育,72小时能明显看见T细胞成团增殖,但是流式检测显示大部分细胞死亡,且CFSE未出现增殖的峰,是什么原因 Click here👆to get an answer to your question ️ calculatecfse of the following complexes fecn64
Here, cu is in d9 system and n h 3 is a low spin ligand, hence, electrons pairing will happen in t2g level ∴ cfse = 3 5×3− 2 5×6 =−3/5δo =−0.6δo was this answer helpful? 荧光染料CFSE (CFDA-SE),是一种可对活细胞进行荧光标记的新型染料,可以标记活体细胞。其基本原理如下:CFSE能够轻易穿透细胞膜,在活细胞内与胞内蛋白共价结合,水解后释放出绿色荧光。在细胞分裂 If δ0 is the octahedral crystal field splitting energy
Then the cfse for f e(cn)6]4− is
(ignore pairing energy) view solution